ALKALINE PHOSPHATASE (AP), ORIGIN BOVINE INTESTINE, RECOMBINANT
phosphatase alkaline from E. coli
CAS: 9001-78-9
Molecular Formula: C21H36N8O6
ALKALINE PHOSPHATASE (AP), ORIGIN BOVINE INTESTINE, RECOMBINANT - Names and Identifiers
ALKALINE PHOSPHATASE (AP), ORIGIN BOVINE INTESTINE, RECOMBINANT - Physico-chemical Properties
| Molecular Formula | C21H36N8O6 |
| Molar Mass | 496.56054 |
| Density | 1.12g/mLat 20°C |
| Water Solubility | It is soluble in water. |
| Appearance | suspension |
| Specific Gravity | 1 |
| Color | white |
| Storage Condition | -20°C |
| Stability | Stability Incompatible with strong oxidizing agents |
| MDL | MFCD00131849 |
| Physical and Chemical Properties | White freeze-dried powder or 3.2mol/L ammonium sulfate suspension, soluble in water and dilute buffer, pI5.7, optimum pH 8.0-10.5 (depending on substrate). Stability: The suspension was stored at 4 ℃ for 6 months with unchanged activity. Freeze-dried powder was stored at 0 ℃ for one year in a calcium chloride vacuum dryer, with 40% loss of vitality. Inhibitors include chelating agents of divalent metal ions (EDTA, oxalate, cysteine, histidine, citric acid), ammonium monoethoxide, inorganic phosphorus, Be2 +, aromatic amino acids, urea, iodoacetamide, L-high arginine, high concentration of Zn2 +. Activators include Tris buffer, low concentration Zn2 +, amino alcohol (2-amino -2-methyl, 1-propanol, diethanolamine), divalent metal ions (Mg2 +, Co2 +, Mn22 +). The absorption coefficient of 1% aqueous solution at 278nm is 7.6. Enzymatic reaction: 4-nitrophenyl phosphate + H_2O_R_A_4-nitrophenol + inorganic phosphorus. |
| Use | This product is for scientific research only and shall not be used for other purposes. |
ALKALINE PHOSPHATASE (AP), ORIGIN BOVINE INTESTINE, RECOMBINANT - Risk and Safety
| Risk Codes | 36/37/38 - Irritating to eyes, respiratory system and skin. |
| Safety Description | S26 - In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. S36 - Wear suitable protective clothing. S24/25 - Avoid contact with skin and eyes. S22 - Do not breathe dust. S23 - Do not breathe vapour. |
| WGK Germany | 3 |
| FLUKA BRAND F CODES | 3-10 |
| TSCA | Yes |
| HS Code | 35079090 |
| Toxicity | An enzyme with an alkaline pH optimum that hydrolyzes phosphate monoesters. Elevation of its activity in the serum usually indicates obstructive jaundice, Paget’s disease (osteitis deformans), or bone carcinoma. |
ALKALINE PHOSPHATASE (AP), ORIGIN BOVINE INTESTINE, RECOMBINANT - Introduction
Alkaline phosphatase can catalyze the removal of DNA, RNA, riboside triphosphate and 5 ′ phosphate groups of deoxyriboside triphosphate. Since the CIP-treated DNA fragments lack the 5 ′ phosphate terminal required by the ligase, they cannot perform self-ligation (1). This property can reduce the background of vector DNA during cloning.
Last Update：2022-10-16 17:29:45
ALKALINE PHOSPHATASE (AP), ORIGIN BOVINE INTESTINE, RECOMBINANT - Source
Calf intestine
Last Update：2023-08-16 21:32:38
ALKALINE PHOSPHATASE (AP), ORIGIN BOVINE INTESTINE, RECOMBINANT - Traits (the following information is for reference only)
Suspension.
Last Update：2023-08-16 21:32:38
ALKALINE PHOSPHATASE (AP), ORIGIN BOVINE INTESTINE, RECOMBINANT - Reference Information
| EPA chemical substance information | information provided by: ofmpeb.epa.gov (external link) |
| Introduction | Alkaline phosphatase (ALP or ALKP) (EC3.1.3.1) is a kind of hydrolase, phosphate groups can be removed from nucleotides, proteins, alkaloids and other molecules for dephosphorylation, which is most effective in alkaline environment, hence the name alkaline phosphatase. |
| function | alkaline phosphatase (ALP) is an extracellular enzyme of osteoblasts, its expression activity is an obvious characteristic of osteoblast differentiation. The main physiological function of alkaline phosphatase in the body is the hydrolysis of phosphate in the process of osteogenesis, which provides the necessary phosphate for the deposition of hydroxyapatite, release of its inhibition of bone mineral formation, is conducive to the role of bone formation. |
| Use | alkaline phosphatase has broad specificity for the phosphate esters of alcohol, Amine, Pyrophosphate and phenol, and it requires zinc and magnesium or calcium divalent ions for activity. It can be used to dephosphorylate casein and other proteins; It can also be used to dephosphorylate the 5 '-end of DNA or RNA to prevent self-ligation. After dephosphorylation with it, DNA or RNA can also be labeled with radiolabeled phosphate (via T4 polynucleotide kinase). It is used for conjugation with antibodies and other proteins for ELISA,Western blotting and Histochemical detection; It is commonly used for dephosphorylation of proteins (e. G. Casein) and nucleic acids. When high sensitivity is required, it can be used for protein labeling. used in biochemical studies, applicable to the enzyme immunoassay technique of the marker, the medicine is commonly used as a diagnostic enzyme. used in industrial and mining enterprises and civil |
| physiological properties | alkaline phosphatase is an enzyme that dephosphorylates the corresponding substrate, that is, the phosphate group on the substrate molecule is removed by hydrolysis of the phosphate monoester, and phosphate ions and free hydroxyl groups are generated. Such substrates include nucleic acids, proteins, alkaloids, and the like. This process of dephosphorylation is referred to as dephosphorylation or dephosphorylation. Alkaline phosphatase is one of the phosphatases, the action of which is opposite to that of kinases, which are phosphorylases, and can use energy molecules, such as ATP, to add phosphate groups to the corresponding substrate molecules. Alkaline phosphatase has the greatest activity in alkaline environment, and its optimum pH is 8.0 for ALP derived from bacteria and 8.5 for ALP derived from cattle. |
| production method | with calf small intestinal mucosa as raw material, homogenate, filtered acid precipitation, extracted with n-butanol, ether and acetone precipitation, and then treated with activated carbon refined products. |
Last Update：2024-04-09 02:00:12
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Product Name: phosphatase alkaline from E. coli Request for quotationCAS: 9001-78-9
Tel: +86-17551318830
Email: r@reformchem.com
Mobile: +86-17551318830
QQ: 3787852685
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